Docosahexaenoic Acid Controls Pulmonary Macrophage Lipid Raft Size and Inflammation.

ABSTRACT:

Background

Docosahexaenoic acid (DHA) controls the biophysical organization of plasma membrane sphingolipid/cholesterol-enriched lipid rafts to exert anti-inflammatory effects, particularly in lymphocytes. However, the impact of DHA on the spatial arrangement of alveolar macrophage lipid rafts and inflammation is unknown.

Objectives

The primary objective was to determine how DHA controls lipid raft organization and function of alveolar macrophages. As proof-of-concept, we also investigated DHA's anti-inflammatory effects on select pulmonary inflammatory markers with a murine influenza model.

Methods

MH-S cells, an alveolar macrophage line, were treated with 50 μM DHA or vehicle control and were used to study plasma membrane molecular organization with fluorescence-bas

SUBMITTER: Pennington ER 

PROVIDER: S-EPMC11217028 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature