Diffusion-driven fed-batch fermentation in perforated ring flasks.

ABSTRACT:

Purpose

Simultaneous membrane-based feeding and monitoring of the oxygen transfer rate shall be introduced to the newly established perforated ring flask, which consists of a cylindrical glass flask with an additional perforated inner glass ring, for rapid bioprocess development.

Methods

A 3D-printed adapter was constructed to enable monitoring of the oxygen transfer rate in the perforated ring flasks. Escherichia coli experiments in batch were performed to validate the adapter. Fed-batch experiments with different diffusion rates and feed solutions were performed.

Results

The adapter and the performed experiments allowed a direct comparison of the perforated ring flasks with Erlenmeyer flasks. In batch cultivations, maximum oxygen transfer capacities of 80 mmol L

SUBMITTER: Luchtrath C 

PROVIDER: S-EPMC11217090 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature