Ultrafast bisulfite sequencing detection of 5-methylcytosine in DNA and RNA.

ABSTRACT: Bisulfite sequencing (BS-seq) to detect 5-methylcytosine (5mC) is limited by lengthy reaction times, severe DNA damage, overestimation of 5mC level and incomplete C-to-U conversion of certain DNA sequences. We present ultrafast BS-seq (UBS-seq), which uses highly concentrated bisulfite reagents and high reaction temperatures to accelerate the bisulfite reaction by ~13-fold, resulting in reduced DNA damage and lower background noise. UBS-seq allows library construction from small amounts of purified genomic DNA, such as from cell-free DNA or directly from 1 to 100 mouse embryonic stem cells, with less overestimation of 5mC level and higher genome coverage than conventional BS-seq. Additionally, UBS-seq quantitatively maps RNA 5-methylcytosine (m⁵C) from low inputs of mRNA and all

SUBMITTER: Dai Q 

PROVIDER: S-EPMC11217147 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature