Programmable protein expression using a genetically encoded m⁶A sensor.

ABSTRACT: The N⁶-methyladenosine (m⁶A) modification is found in thousands of cellular mRNAs and is a critical regulator of gene expression and cellular physiology. m⁶A dysregulation contributes to several human diseases, and the m⁶A methyltransferase machinery has emerged as a promising therapeutic target. However, current methods for studying m⁶A require RNA isolation and do not provide a real-time readout of mRNA methylation in living cells. Here we present a genetically encoded m⁶A sensor (GEMS) technology, which couples a fluorescent signal with cellular mRNA methylation. GEMS detects changes in m⁶A caused by pharmacological inhibition of the m⁶A methyltransferase, giving it potential utility for drug discove

SUBMITTER: Marayati BF 

PROVIDER: S-EPMC11217150 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature