Label-free morpho-molecular phenotyping of living cancer cells by combined Raman spectroscopy and phase tomography.

ABSTRACT: Accurate, rapid and non-invasive cancer cell phenotyping is a pressing concern across the life sciences, as standard immuno-chemical imaging and omics require extended sample manipulation. Here we combine Raman micro-spectroscopy and phase tomography to achieve label-free morpho-molecular profiling of human colon cancer cells, following the adenoma, carcinoma, and metastasis disease progression, in living and unperturbed conditions. We describe how to decode and interpret quantitative chemical and co-registered morphological cell traits from Raman fingerprint spectra and refractive index tomograms. Our multimodal imaging strategy rapidly distinguishes cancer phenotypes, limiting observations to a low number of pristine cells in culture. This synergistic dataset allows us to study independe

SUBMITTER: Bresci A 

PROVIDER: S-EPMC11217291 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature