SUPRa is a dual-color reporter for unbiased quantification of the unfolded protein response with cellular resolution.

ABSTRACT: The unfolded protein response (UPR) maintains proteostasis upon endoplasmic reticulum (ER) stress, and is initiated by a range of physiological and pathological processes. While there have been advances in developing fluorescent reporters for monitoring individual signaling pathways of the UPR, this approach may not capture a cell's overall UPR activity. Here we describe a novel sensor of UPR activity, sUPRa, which is designed to report the global UPR. sUPRa displays excellent response characteristics, outperforms reporters of individual UPR pathways in terms of sensitivity and kinetics, and responds to a range of different ER stress stimuli. Furthermore, sUPRa's dual promoter and fluorescent protein design ensures that both UPR-active and inactive cells are detected, and controls for repo

SUBMITTER: Chakrabarty A 

PROVIDER: S-EPMC11217371 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature