Rapid evolution of colistin resistance in a bioreactor model of infection of Klebsiella pneumoniae.

ABSTRACT: Colistin remains an important antibiotic for the therapeutic management of drug-resistant Klebsiella pneumoniae. Despite the numerous reports of colistin resistance in clinical strains, it remains unclear exactly when and how different mutational events arise resulting in reduced colistin susceptibility. Using a bioreactor model of infection, we modelled the emergence of colistin resistance in a susceptible isolate of K. pneumoniae. Genotypic, phenotypic and mathematical analyses of the antibiotic-challenged and un-challenged population indicates that after an initial decline, the population recovers within 24 h due to a small number of "founder cells" which have single point mutations mainly in the regulatory genes encoding crrB and pmrB that when mutated results in up to 100-fold reducti

SUBMITTER: Jimenez-Castellanos JC 

PROVIDER: S-EPMC11217424 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature