Circadian regulation of macromolecular complex turnover and proteome renewal.

ABSTRACT: Although costly to maintain, protein homeostasis is indispensable for normal cellular function and long-term health. In mammalian cells and tissues, daily variation in global protein synthesis has been observed, but its utility and consequences for proteome integrity are not fully understood. Using several different pulse-labelling strategies, here we gain direct insight into the relationship between protein synthesis and abundance proteome-wide. We show that protein degradation varies in-phase with protein synthesis, facilitating rhythms in turnover rather than abundance. This results in daily consolidation of proteome renewal whilst minimising changes in composition. Coupled rhythms in synthesis and turnover are especially salient to the assembly of macromolecular protein complexes, part

SUBMITTER: Seinkmane E 

PROVIDER: S-EPMC11217436 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature