Elucidating immune-related gene transcriptional programs via factorization of large-scale RNA-profiles.

ABSTRACT: Recent developments in immunotherapy, including immune checkpoint blockade (ICB) and adoptive cell therapy (ACT), have encountered challenges such as immune-related adverse events and resistance, especially in solid tumors. To advance the field, a deeper understanding of the molecular mechanisms behind treatment responses and resistance is essential. However, the lack of functionally characterized immune-related gene sets has limited data-driven immunological research. To address this gap, we adopted non-negative matrix factorization on 83 human bulk RNA sequencing (RNA-seq) datasets and constructed 28 immune-specific gene sets. After rigorous immunologist-led manual annotations and orthogonal validations across immunological contexts and functional omics data, we demonstrated that these g

SUBMITTER: He S 

PROVIDER: S-EPMC11217617 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature