Addressing bioreactor hiPSC aggregate stability, maintenance and scaleup challenges using a design of experiment approach.

ABSTRACT:

Background

Stem cell-derived therapies hold the potential for treatment of regenerative clinical indications. Static culture has a limited ability to scale up thus restricting its use. Suspension culturing can be used to produce target cells in large quantities, but also presents challenges related to stress and aggregation stability.

Methods

Utilizing a design of experiments (DoE) approach in vertical wheel bioreactors, we evaluated media additives that have versatile properties. The additives evaluated are Heparin sodium salt (HS), polyethylene glycol (PEG), poly (vinyl alcohol) (PVA), Pluronic F68 and dextran sulfate (DS). Multiple response variables were chosen to assess cell growth, pluripotency maintenance and aggregate stability in response to the additive inputs, and

SUBMITTER: Yehya H 

PROVIDER: S-EPMC11218057 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature