An expedited screening platform for the discovery of anti-ageing compounds in vitro and in vivo.

ABSTRACT:

Background

Restraining or slowing ageing hallmarks at the cellular level have been proposed as a route to increased organismal lifespan and healthspan. Consequently, there is great interest in anti-ageing drug discovery. However, this currently requires laborious and lengthy longevity analysis. Here, we present a novel screening readout for the expedited discovery of compounds that restrain ageing of cell populations in vitro and enable extension of in vivo lifespan.

Methods

Using Illumina methylation arrays, we monitored DNA methylation changes accompanying long-term passaging of adult primary human cells in culture. This enabled us to develop, test, and validate the CellPopAge Clock, an epigenetic clock with underlying algorithm, unique among existing epigenetic clocks for

SUBMITTER: Lujan C 

PROVIDER: S-EPMC11218148 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature