CRISPR-based genome editing of a diurnal rodent, Nile grass rat (Arvicanthis niloticus).

ABSTRACT:

Background

Diurnal and nocturnal mammals have evolved distinct pathways to optimize survival for their chronotype-specific lifestyles. Conventional rodent models, being nocturnal, may not sufficiently recapitulate the biology of diurnal humans in health and disease. Although diurnal rodents are potentially advantageous for translational research, until recently, they have not been genetically tractable. The present study aims to address this major limitation by developing experimental procedures necessary for genome editing in a well-established diurnal rodent model, the Nile grass rat (Arvicanthis niloticus).

Results

A superovulation protocol was established, which yielded nearly 30 eggs per female grass rat. Fertilized eggs were cultured in a modified rat 1-cell embryo cul

SUBMITTER: Xie H 

PROVIDER: S-EPMC11218167 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature