Single-cell transcriptomic profiling unveils insights into ovarian fibrosis in obese mice.

ABSTRACT:

Background

Adiposity profoundly impacts reproductive health in both humans and animals. However, the precise subpopulations contributing to infertility under obese conditions remain elusive.

Results

In this study, we established an obese mouse model through an eighteen-week high-fat diet regimen in adult female mice. Employing single-cell RNA sequencing (scRNA-seq), we constructed a comprehensive single-cell atlas of ovarian tissues from these mice to scrutinize the impact of obesity on the ovarian microenvironment. ScRNA-seq revealed notable alterations in the microenvironment of ovarian tissues in obese mice. Granulosa cells, stromal cells, T cells, and macrophages exhibited functional imbalances compared to the control group. We observed heightened interaction strength in

SUBMITTER: Xiao B 

PROVIDER: S-EPMC11218254 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature