Streamlining heterologous expression of top carbonic anhydrases in Escherichia coli: bioinformatic and experimental approaches.

ABSTRACT:

Background

Carbonic anhydrase (CA) enzymes facilitate the reversible hydration of CO₂ to bicarbonate ions and protons. Identifying efficient and robust CAs and expressing them in model host cells, such as Escherichia coli, enables more efficient engineering of these enzymes for industrial CO₂ capture. However, expression of CAs in E. coli is challenging due to the possible formation of insoluble protein aggregates, or inclusion bodies. This makes the production of soluble and active CA protein a prerequisite for downstream applications.

Results

In this study, we streamlined the process of CA expression by selecting seven top CA candidates and used two bioinformatic tools to predict their solubility for expression in E. coli. The prediction results place

SUBMITTER: Wei H 

PROVIDER: S-EPMC11218372 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature