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NOX4-mediated astrocyte ferroptosis in Alzheimer's disease.

ABSTRACT: This study investigates NADPH oxidase 4 (NOX4) involvement in iron-mediated astrocyte cell death in Alzheimer's Disease (AD) using single-cell sequencing data and transcriptomes. We analyzed AD single-cell RNA sequencing data, identified astrocyte marker genes, and explored biological processes in astrocytes. We integrated AD-related chip data with ferroptosis-related genes, highlighting NOX4. We validated NOX4's role in ferroptosis and AD in vitro and in vivo. Astrocyte marker genes were enriched in AD, emphasizing their role. NOX4 emerged as a crucial player in astrocytic ferroptosis in AD. Silencing NOX4 mitigated ferroptosis, improved cognition, reduced Aβ and p-Tau levels, and alleviated mitochondrial abnormalities. NOX4 promotes astrocytic ferroptosis, underscoring its significance in AD progression.

SUBMITTER: Maimaiti Y

PROVIDER: S-EPMC11218381 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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NOX4-mediated astrocyte ferroptosis in Alzheimer's disease.

Maimaiti Yasenjiang Y Su Ting T Zhang Zhanying Z Ma Lingling L Zhang Yuan Y Xu Hong H

Cell & bioscience 20240702 1

This study investigates NADPH oxidase 4 (NOX4) involvement in iron-mediated astrocyte cell death in Alzheimer's Disease (AD) using single-cell sequencing data and transcriptomes. We analyzed AD single-cell RNA sequencing data, identified astrocyte marker genes, and explored biological processes in astrocytes. We integrated AD-related chip data with ferroptosis-related genes, highlighting NOX4. We validated NOX4's role in ferroptosis and AD in vitro and in vivo. Astrocyte marker genes were enrich ...[more]

PMID: 38956702

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