Mouse model of radiation retinopathy reveals vascular and neuronal injury.

ABSTRACT:

Purpose

To characterize the neuronal and vascular pathology in vivo and in vitro in a mouse model of radiation retinopathy.

Methods

C57Bl/6J mice underwent cranial irradiation with 12 Gy and in vivo imaging by optical coherence tomography and of relative blood flow velocity by laser speckle flowgraphy for up to 3-6 months after irradiation. Retinal architecture, vascular density and leakage and apoptosis were analyzed by histology and immunohistochemistry before irradiation or at 10, 30, 240, and 365 days after treatment.

Results

The vascular density decreased in the plexiform layers starting at 30 days after irradiation. No impairment in retinal flow velocity was seen. Subtle perivascular leakage was present at 10 days, in particular in the outer plexiform layer. Thi

SUBMITTER: Liu E 

PROVIDER: S-EPMC11218432 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature