Characterization of two affinity matured Anti-Yersinia pestis F1 human antibodies with medical countermeasure potential.

ABSTRACT: Yersinia pestis, the causative agent of plague and a biological threat agent, presents an urgent need for novel medical countermeasures due to documented cases of naturally acquired antibiotic resistance and potential person-to-person spread during a pneumonic infection. Immunotherapy has been proposed as a way to circumvent current and future antibiotic resistance. Here, we describe the development and characterization of two affinity matured human antibodies (αF1Ig AM2 and αF1Ig AM8) that promote survival of mice after exposure to aerosolized Y. pestis. We share details of the error prone PCR and yeast display technology-based affinity maturation process that we used. The resultant matured antibodies have nanomolar affinity for Y. pestis F1 antigen, are produced in high yield, and are re

SUBMITTER: Velappan N 

PROVIDER: S-EPMC11218954 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature