Advantages of stereolithographic 3D printing in the fabrication of the Affiblot device for dot-blot assays.

ABSTRACT: In stereolithographic (SLA) 3D printing, objects are constructed by exposing layers of photocurable resin to UV light. It is a highly user-friendly fabrication method that opens a possibility for technology sharing through CAD file online libraries. Here, we present a prototyping procedure of a microfluidics-enhanced dot-blot device (Affiblot) designed for simple and inexpensive screening of affinity molecule characteristics (antibodies, oligonucleotides, cell receptors, etc.). The incorporation of microfluidic features makes sample processing user-friendly, less time-consuming, and less laborious, all performed completely on-device, distinguishing it from other dot-blot devices. Initially, the Affiblot device was fabricated using CNC machining, which required significant investment in man

SUBMITTER: Novotny J 

PROVIDER: S-EPMC11219379 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature