A crustacean neuropeptide spectral library for data-independent acquisition (DIA) mass spectrometry applications.

ABSTRACT: Neuropeptides have tremendous potential for application in modern medicine, including utility as biomarkers and therapeutics. To overcome the inherent challenges associated with neuropeptide identification and characterization, data-independent acquisition (DIA) is a fitting mass spectrometry (MS) method of choice to achieve sensitive and accurate analysis. It is advantageous for preliminary neuropeptidomic studies to occur in less complex organisms, with crustacean models serving as a popular choice due to their relatively simple nervous system. With spectral libraries serving as a means to interpret DIA-MS output spectra, and Cancer borealis as a model of choice for neuropeptide analysis, we performed the first spectral library mapping of crustacean neuropeptides. Leveraging pre-existing

SUBMITTER: Fields L 

PROVIDER: S-EPMC11219527 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature