High-dimensional mapping of human CEACAM1 expression on immune cells and association with melanoma drug resistance.

ABSTRACT:

Background

Human carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) is an inhibitory cell surface protein that functions through homophilic and heterophilic ligand binding. Its expression on immune cells in human tumors is poorly understood.

Methods

An antibody that distinguishes human CEACAM1 from other highly related CEACAM family members was labeled with ¹⁵⁹Tb and inserted into a panel of antibodies that included specificity for programmed cell death protein 1 (PD1) and PD-L1, which are targets of immunotherapy, to gain a data-driven immune cell atlas using cytometry by time-of-flight (CyTOF). A detailed inventory of CEACAM1, PD1, and PD-L1 expression on immune cells in metastatic lesions to lymph node or soft tissues and peripheral blood samples from

SUBMITTER: Huang YH 

PROVIDER: S-EPMC11219841 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature