Systematic in vitro optimization of antimicrobial peptides against Escherichia coli.

ABSTRACT:

Objectives

Antimicrobial resistance is a growing concern and claims over 1 million lives per year. The discovery of new antimicrobial drugs is expensive and often generates low profitability, with very low success rates. One way to combat this is by the improvement of known antimicrobials, such as antimicrobial peptides (AMPs). The aim of this study was to improve the antimicrobial activities of two known AMPs, UyCT3 and indolicidin, with the use of peptide libraries and growth curves.

Methods

Peptide permutation libraries were synthesized for two AMPs, indolicidin and UyCT3, which included 520 peptides. These peptides were subsequently tested against MG1655-K12, to which subsequent peptide design was performed, then tested against three clinically Gram-negative relevant dru

SUBMITTER: Shukri A 

PROVIDER: S-EPMC11220656 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature