A proof-of-principle study for the point-of-care detection of ESBL (CTX-M) by NG-Test^® CTX-M MULTI lateral flow assay in urine samples using a simplified method for use in a resource-limited setting.

ABSTRACT:

Background

The rise of extended-spectrum β-lactamase-producing Enterobacterales (ESBL-E) in low- and middle-income countries limits treatment options, leading to the frequent use of broad-spectrum antibiotics. Reducing time-to-result for a urinary infection can facilitate correct antibiotic treatment and support antimicrobial and diagnostic stewardship measures. This study compared two simplified enrichment methods for detecting CTX-M directly from urine specimens.

Methods

Two enrichment methods, namely centrifugation of 2 mL urine and filtration of 1 mL urine using the DirecTool adaptor, were compared using 20 culture-positive urine samples (20 suspected ESBL-E and 20 non-ESBL-E). CTX-M production was detected using a lateral flow assay (LFA), NG-Test^® CTX-MMULTI

SUBMITTER: Nurjadi D 

PROVIDER: S-EPMC11220667 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature