Nobody's Perfect: Choice of the Buffer and the Rate of Cu²⁺ Ion-Peptide Interaction.

ABSTRACT: The choice of correct pH buffer is crucial in chemical studies modeling biological processes involving Cu²⁺ ions. Popular buffers for physiological pH are known to form Cu(II) complexes, but their impact on kinetics of Cu(II) complexation has not been considered. We performed a stopped-flow kinetic study of Cu²⁺ ion interactions with four popular buffers (phosphate, Tris, HEPES, and MOPS) and two buffers considered as nonbinding (MES and PIPPS). Next, we studied their effects on the rate of Cu²⁺ reaction with Gly-Gly-His (GGH), a tripeptide modeling physiological Cu(II) sites, which we studied previously at conditions presumably excluding the buffer interference [Kotuniak, R.; Angew. Chem., Int. Ed. 2020, 59, 11234-11239]. We observed that (i) all te

SUBMITTER: Kotuniak R 

PROVIDER: S-EPMC11220749 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature