A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves.

ABSTRACT:

Background

Optimization of a highly efficient transient expression system is critical for the study of gene function, particularly in those plants in which stable transformation methods are not widely available. Agrobacterium tumefaciens‑mediated transient transformation is a simple and low-cost method that has been developed and applied to a wide variety of plant species. However, the transient expression in spinach (Spinacia oleracea L.) is still not reported.

Results

We developed a transient expression system in spinach leaves of the Sp75 and Sp73 varieties. Several factors influencing the transformation efficiency were optimized such as Agrobacterium strain, spinach seedling stage, leaf position, and the expression time after injection. Agrobacterium strain GV3101 (pSoup

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11220957 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature