Discovery of Borosin Catalytic Strategies and Function through Bioinformatic Profiling.

ABSTRACT: Borosins are ribosomally synthesized and post-translationally modified peptides (RiPPs) containing backbone α-N-methylations. These modifications confer favorable pharmacokinetic properties including increased membrane permeability and resistance to proteolytic degradation. Previous studies have biochemically and bioinformatically explored several borosins, revealing (1) numerous domain architectures and (2) diverse core regions lacking conserved sequence elements. Due to these characteristics, large-scale computational identification of borosin biosynthetic genes remains challenging and often requires additional, time-intensive manual inspection. This work builds upon previous findings and updates the genome-mining tool RODEO to automatically evaluate borosin biosynthetic gene clus

SUBMITTER: Lee AR 

PROVIDER: S-EPMC11221551 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature