Regenerative capacity of human pluripotent stem cell-derived articular chondrocytes in vitro.

ABSTRACT: The ideal cell source for articular cartilage repair remains elusive. Using developmentally inspired differentiation protocols, we induced human pluripotent stem cells (hPSCs) toward articular chondrocytes capable of joint cartilage repair in rodent models, which were distinct from growth plate chondrocytes, fated to be replaced by bone in vivo. Working toward clinical translation, we demonstrated controlled differentiation into chondrocytes by comprehensive gene expression analysis at each step of the differentiation. Articular chondrocytes derived from hPSCs could be expanded several passages in vitro without losing chondrogenic potential. Furthermore, chondrocytes isolated from these articular cartilage tissues had the potential to serially regenerate new articular and growth plate cart

SUBMITTER: Raftery RM 

PROVIDER: S-EPMC11222035 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature