Quantification of cardiac capillarization in basement-membrane-immunostained myocardial slices using Segment Anything Model.

ABSTRACT: Decreased myocardial capillary density has been reported as an important histopathological feature associated with various heart disorders. Quantitative assessment of cardiac capillarization typically involves double immunostaining of cardiomyocytes (CMs) and capillaries in myocardial slices. In contrast, single immunostaining of basement membrane protein is a straightforward approach to simultaneously label CMs and capillaries, presenting fewer challenges in background staining. However, subsequent image analysis always requires expertise and laborious manual work to identify and segment CMs/capillaries. Here, we developed an image analysis tool, AutoQC, for automatic identification and segmentation of CMs and capillaries in immunofluorescence images of basement membrane. Commonly used ca

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC11222533 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature