Analysis of culture and RNA isolation methods for precision-cut liver slices from cirrhotic rats.

ABSTRACT: Precision-cut liver slices (PCLS) are increasingly used as a model to investigate anti-fibrotic therapies. However, many studies use PCLS from healthy animals treated with pro-fibrotic stimuli in culture, which reflects only the early stages of fibrosis. The effects of different culture conditions on PCLS from cirrhotic animals has not been well characterized and there is no consensus on optimal methods. In this study, we report a method for the collection and culture of cirrhotic PCLS and compare the effect of common culture conditions on viability, function, and gene expression. Additionally, we compared three methods of RNA isolation and identified a protocol with high yield and purity. We observed significantly increased albumin production when cultured with insulin-transferrin-seleniu

SUBMITTER: Leaker BD 

PROVIDER: S-EPMC11222550 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature