Disruptive effects of plasticizers bisphenol A, F, and S on steroidogenesis of adrenocortical cells.

ABSTRACT:

Introduction

Endocrine disrupting chemicals (EDCs) are known to interfere with endocrine homeostasis. Their impact on the adrenal cortex and steroidogenesis has not yet been sufficiently elucidated. This applies in particular to the ubiquitously available bisphenols A (BPA), F (BPF), and S (BPS).

Methods

NCI-H295R adrenocortical cells were exposed to different concentrations (1nM-1mM) of BPA, BPF, BPS, and an equimolar mixture of them (BPmix). After 72 hours, 15 endogenous steroids were measured using LC-MS/MS. Ratios of substrate and product of CYP-regulated steps were calculated to identify most influenced steps of steroidogenesis. mRNA expression of steroidogenic enzymes was determined by real-time PCR.

Results

Cell viability remained unaffected at bisphenol concen

SUBMITTER: Potzl B 

PROVIDER: S-EPMC11222671 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature