Mechanical property estimation of sarcoma-relevant extracellular vesicles using transmission electron microscopy.

ABSTRACT: Analysis of single extracellular vesicles (EVs) has the potential to yield valuable label-free information on their morphological structure, biomarkers and therapeutic targets, though such analysis is hindered by the lack of reliable and quantitative measurements of the mechanical properties of these compliant nanoscale particles. The technical challenge in mechanical property measurements arises from the existing tools and methods that offer limited throughput, and the reported elastic moduli range over several orders of magnitude. Here, we report on a flow-based method complemented by transmission electron microscopy (TEM) imaging to provide a high throughput, whole EV deformation analysis for estimating the mechanical properties of liposarcoma-derived EVs as a function of their size. Ou

SUBMITTER: Singh PK 

PROVIDER: S-EPMC11222873 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature