Interleukin-7-based identification of liver lymphatic endothelial cells reveals their unique structural features.

ABSTRACT:

Background & aims

The lymphatic system plays crucial roles in maintaining fluid balance and immune regulation. Studying the liver lymphatics has been considered challenging, as common lymphatic endothelial cell (LyEC) markers are expressed by other liver cells. Additionally, isolation of sufficient numbers of LyECs has been challenging because of their extremely low abundance (<0.01% of entire liver cell population) in a normal liver.

Methods

Potential LyEC markers was identified using our published single-cell RNA sequencing (scRNA-seq) dataset (GSE147581) in mouse livers. Interleukin-7 (IL7) promoter-driven green fluorescent protein knock-in heterozygous mice were used for the validation of IL7 expression in LyECs in the liver, for the development of liver LyEC isolation p

SUBMITTER: Yang Y 

PROVIDER: S-EPMC11222939 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature