Assessing seromuscular layer and serosa removal on intestinal permeability measurements in weaned piglet everted sac segments.

ABSTRACT: The integrity of the intestinal barrier is crucial for regulating the passage of pathogens and toxins, while facilitating nutrient absorption. The everted gut sac technique, an ex-vivo technique, can be used to study interventions on barrier function. This cost-effective approach utilizes relatively large gut segments to study specific intestinal regions. Typically, intact (non-stripped) intestinal segments are used, but their use may underestimate permeability due to the medial positioning of blood vessels relative to the seromuscular layer and serosa. However, removing these layers risks physical damage, resulting in an overestimation of intestinal permeability. Therefore, we investigated the impact of stripping jejunal segments on permeability to fluorescein isothiocyanate-dextran (FITC

SUBMITTER: Noorman L 

PROVIDER: S-EPMC11222984 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature