Sensitive Detection and Quantification of Oxygenated Compounds in Complex Samples Using GC-Combustion-MS.

ABSTRACT: This work introduces a new element-selective gas chromatography detector for the accurate quantification of traces of volatile oxygen-containing compounds in complex samples without the need for specific standards. The key to this approach is the use of oxygen highly enriched in ¹⁸O as the oxidizing gas in a combustion unit (800 °C) that allows us to directly and unambiguously detect the natural oxygen present in the GC-separated compounds through its incorporation into the volatile species formed after their combustion and their subsequent degradation to ¹⁶O in the ion source. The unspecific signal due to the low ¹⁶O abundance in the oxidizing gas could be compensated by measuring the m/z 12 that comes as well from the CO₂ degradat

SUBMITTER: Garcia-Bellido J 

PROVIDER: S-EPMC11223096 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature