Dataset Information

Carbohydrate antigen 125 on epicardial fat and its association with local inflammation and fibrosis-related markers.

ABSTRACT:

Background

Carbohydrate antigen 125 (CA125) is a proteolytic fragment of MUC-16 that is increased in heart failure (HF) and associated with inflammation, fluid overload, and worse adverse events. Our main objective was to study the expression of CA125 on epicardium and its association with inflammation, adipogenesis, and fibrosis.

Methods

Epicardial fat biopsies and blood were obtained from 151 non-selected patients undergoing open heart surgery. Immunohistochemistry, ELISA, or real-time PCR were used for analyzing protein or mRNA expression levels of CA125 and markers of inflammatory cells, fibroblasts, and adipocytes. Epithelial or stromal cells from epicardium were isolated and cultured to identify CA125 and its association with the adipogenesis and fibrosis pathways, respectively.

Results

The median age was 71 (63-74) years, 106 patients (70%) were male, and 62 (41%) had an established diagnosis of HF before surgery. The slice of epicardial fat biopsy determined a positive and colorimetric staining on the epithelial layer after incubating with the CA125 M11 antibody, providing the first description of CA125 expression in the human epicardium. Epicardial CA125 showed a strong and positive correlation with markers of inflammation and fibrosis in the epicardial fat tissue while exhibiting a negative correlation with markers of the adipogenesis pathway. This relationship remained significant after adjusting for potential confounders such as a prior HF diagnosis and plasma CA125 levels.

Conclusion

Epicardial cells express CA125, which is positively associated with inflammatory and fibroblast markers in epicardial adipose tissue. These results suggest that CA125 may be biologically involved in HF progression (transition from adipogenesis to fibrosis).

SUBMITTER: Eiras S

PROVIDER: S-EPMC11223376 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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Publications

Carbohydrate antigen 125 on epicardial fat and its association with local inflammation and fibrosis-related markers.

Eiras Sonia S de la Espriella Rafael R Fu Xiaoran X Iglesias-Álvarez Diego D Basdas Rumeysa R Núñez-Caamaño J R JR Martínez-Cereijo J M JM Reija L L Fernández A L AL Sánchez-López David D Miñana Gema G Núñez Julio J González-Juanatey José R JR

Journal of translational medicine 20240703 1

<h4>Background</h4>Carbohydrate antigen 125 (CA125) is a proteolytic fragment of MUC-16 that is increased in heart failure (HF) and associated with inflammation, fluid overload, and worse adverse events. Our main objective was to study the expression of CA125 on epicardium and its association with inflammation, adipogenesis, and fibrosis.<h4>Methods</h4>Epicardial fat biopsies and blood were obtained from 151 non-selected patients undergoing open heart surgery. Immunohistochemistry, ELISA, or re ...[more]

PMID: 38961436

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Project description:Simple Summary Endometrial cancer is a common gynecological malignancy, many patients have early clinical symptoms. However, disease progression and even death can occur in some cases after initial treatment. Identifying convenient and effective markers to predict the prognosis of patients with endometrial cancer and guide the selection of treatment options and patient management is urgently needed. In this study, the fibrinogen, albumin, NLR, and CA125 levels as well as other clinicopathological features of patients with endometrial cancer were recorded. Nomograms for predicting endometrial cancer prognosis created by combining fibrinogen, albumin, NLR, and CA125 levels were constructed, verified and evaluated, and the nomograms are highly accurate and have clinical application value, which provided a convenient method for predicting the prognosis of patients undergoing surgical treatment for endometrial cancer. Abstract Background: This study aimed to determine the prognostic value of the preoperative levels of fibrinogen, albumin (ALB), neutrophil–lymphocyte ratio (NLR), and carbohydrate antigen 125 (CA125) in endometrial cancer and to establish nomograms for predicting patient survival. Methods: Patients with endometrial cancer (n = 1483) who underwent surgery were included in this study, and their preoperative fibrinogen, ALB, NLR, and CA125 levels and clinicopathological characteristics were collected. Patients were randomized into a training cohort (70%, n = 1038) and an external validation cohort (30%, n = 445). The Cox regression analysis was performed using the data for the patients in the training cohort to identify independent prognostic factors; nomograms for predicting prognosis were established and validated. Results: High fibrinogen (≥3.185 g/L), NLR (≥2.521 g/L), and CA125 (≥35 U/mL) levels and low ALB (<4.185 g/L) levels were independently associated with poor progression-free survival (PFS) and poor overall survival (OS) in patients with endometrial cancer. Prognostic prediction model nomograms were developed and validated based on these results. Calibration curves and C-indexes underscored the good predictive power of the nomograms, and both the net reclassification index (NRI) and integrated discrimination improvement (IDI) values of the prognostic prediction model nomograms were improved. Conclusions: Nomograms that are developed based on preoperative fibrinogen, ALB, NLR, and CA125 levels accurately predict PFS and OS in patients with endometrial cancer.

Dataset Information

Carbohydrate antigen 125 on epicardial fat and its association with local inflammation and fibrosis-related markers.

Background

Methods

Results

Conclusion

Publications

Carbohydrate antigen 125 on epicardial fat and its association with local inflammation and fibrosis-related markers.

Similar Datasets

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