A comparison of the TempO-Seq and Affymetrix microarray platform using RTqPCR validation.

ABSTRACT: Next-generation risk assessment relies on mechanistic data from new approach methods, including transcriptome data. Various technologies, such as high-throughput targeted sequencing methods and microarray technologies based on hybridization with complementary probes, are used to determine differentially expressed genes (DEGs). The integration of data from different technologies requires a good understanding of the differences arising from the use of various technologies.To better understand the differences between the TempO-Seq platform and Affymetrix chip technology, whole-genome data for the volatile compound dimethylamine were compared. Selected DEGs were also confirmed using RTqPCR validation. Although the overlap of DEGs between TempO-Seq and Affymetrix was no higher than 37%, a compa

SUBMITTER: Wehr MM 

PROVIDER: S-EPMC11223392 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature