Simultaneous genetic transformation and genome editing of mixed lines in soybean (Glycine max) and maize (Zea mays).

ABSTRACT: Robust genome editing technologies are becoming part of the crop breeding toolbox. Currently, genome editing is usually conducted either at a single locus, or multiple loci, in a variety at one time. Massively parallel genomics platforms, multifaceted genome editing capabilities, and flexible transformation systems enable targeted variation at nearly any locus, across the spectrum of genotypes within a species. We demonstrate here the simultaneous transformation and editing of many genotypes, by targeting mixed seed embryo explants with genome editing machinery, followed by re-identification through genotyping after plant regeneration. Transformation and Editing of Mixed Lines (TREDMIL) produced transformed individuals representing 101 of 104 (97%) mixed elite genotypes in soybean; and 22

SUBMITTER: Valentine M 

PROVIDER: S-EPMC11224177 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature