Identification of human host factors required for beta-defensin-2 expression in intestinal epithelial cells upon a bacterial challenge.

ABSTRACT: The human intestinal tract is colonized with microorganisms, which present a diverse array of immunological challenges. A number of antimicrobial mechanisms have evolved to cope with these challenges. A key defense mechanism is the expression of inducible antimicrobial peptides (AMPs), such as beta-defensins, which rapidly inactivate microorganisms. We currently have a limited knowledge of mechanisms regulating the inducible expression of AMP genes, especially factors from the host required in these regulatory mechanisms. To identify the host factors required for expression of the beta-defensin-2 gene (HBD2) in intestinal epithelial cells upon a bacterial challenge, we performed a RNAi screen using a siRNA library spanning the whole human genome. The screening was performed in duplicate to

SUBMITTER: Wozniak W 

PROVIDER: S-EPMC11224401 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature