HaloTag display enables quantitative single-particle characterisation and functionalisation of engineered extracellular vesicles.

ABSTRACT: Extracellular vesicles (EVs) play key roles in diverse biological processes, transport biomolecules between cells and have been engineered for therapeutic applications. A useful EV bioengineering strategy is to express engineered proteins on the EV surface to confer targeting, bioactivity and other properties. Measuring how incorporation varies across a population of EVs is important for characterising such materials and understanding their function, yet it remains challenging to quantitatively characterise the absolute number of engineered proteins incorporated at single-EV resolution. To address these needs, we developed a HaloTag-based characterisation platform in which dyes or other synthetic species can be covalently and stoichiometrically attached to engineered proteins on the EV sur

SUBMITTER: Mitrut RE 

PROVIDER: S-EPMC11224594 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature