Production of succinate with two CO₂ fixation reactions from fatty acids in Cupriavidus necator H16.

ABSTRACT:

Background

Biotransformation of CO₂ into high-value-added carbon-based products is a promising process for reducing greenhouse gas emissions. To realize the green transformation of CO₂, we use fatty acids as carbon source to drive CO₂ fixation to produce succinate through a portion of the 3-hydroxypropionate (3HP) cycle in Cupriavidus necator H16.

Results

This work can achieve the production of a single succinate molecule from one acetyl-CoA molecule and two CO₂ molecules. It was verified using an isotope labeling experiment utilizing NaH¹³CO₃. This implies that 50% of the carbon atoms present in succinate are derived from CO₂, resulting in a twofold increase in efficiency compared to prior methods

SUBMITTER: Li L 

PROVIDER: S-EPMC11225152 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature