ETV4 promotes the progression of cholangiocarcinoma by regulating glycolysis via the TGF-β signaling.

ABSTRACT:

Background

Considerable studies show that ETS variant 4 (ETV4) plays an important roles in multitudinous tumor. This study investigated its function in cholangiocarcinoma (CCA) progression and revealed the underlying mechanisms.

Methods

The expression of ETV4 in CCA was evaluated using TCGA database and the single-cell analysis based on GSE189903 dataset. ETV4 expression in CCA human specimens was detected by reverse transcription-quantitative PCR, immunohistochemistry, and western blot. Cell Counting Kit-8, EdU, colony formation, wound healing, and Transwell assays were used to analyze the effects of ETV4. Extracellular acidification rate, oxygen consumption rate, glucose uptake, and lactate production were used to measure glycolysis in CAA cells. Western blot was performed

SUBMITTER: Liu F 

PROVIDER: S-EPMC11225894 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature