Time-course study of genetic changes in periodontal ligament regeneration after tooth replantation in a mouse model.

ABSTRACT: This research focused on analyzing gene expression changes in the periodontal ligament (PDL) after tooth re-plantation to identify key genes and pathways involved in healing and regeneration. Utilizing a mouse model, mRNA was extracted from the PDL at various intervals post-replantation for RNA sequencing analysis, spanning from 3 to 56 days. The results revealed significant shifts in gene expression, particularly notable on day 28, supported by hierarchical clustering and principal component analysis. Gene ontology (GO) enrichment analysis highlighted an upregulation in olfactory receptor and G protein-coupled receptor signaling pathways at this time point. These findings were validated through reverse transcription-quantitative PCR (RT-qPCR), with immunochemical staining localizing olfac

SUBMITTER: Ohshima J 

PROVIDER: S-EPMC11226448 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature