Systematic optimization of culture media for maintenance of human induced pluripotent stem cells using the response surface methodology.

ABSTRACT: The application of human induced pluripotent stem cells (hiPSCs) provides tremendous opportunities in cell therapy. However, culturing these cells faces many practical challenges, including costs associated with cell culture media and the optimization of cell culture conditions. Providing an optimized culture platform for hiPSCs to maintain pluripotency and self-renewal and generate cost-effective and robust therapeutics is an immediate requirement. This study used the design of experiments and the response surface methodology, a powerful statistical tool, to generate empirical models for predicting the optimal culture conditions of the hiPSCs. Pluripotency and cell proliferation were applied as read-outs to determine the optimal concentration of basic fibroblast growth factor (bFGF) and c

SUBMITTER: Mousavi Mirkalaei S 

PROVIDER: S-EPMC11226774 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature