Investigation of the human-gut-kidney axis by fecal proteomics, highlights molecular mechanisms affected in CKD.

ABSTRACT:

Objective

The interplay of gut microbiota with the kidney system in chronic kidney disease (CKD), is characterized by increased concentrations of uric acid in the gut, which in turn, may increase bacterial uricase activity and may lead to the generation of uremic toxins. Nevertheless, knowledge on these underlying bidirectional molecular mechanisms is still limited.

Methods

In this exploratory study, proteomic analysis was performed on fecal samples, targeting to investigate this largely unexplored biological material as a source of information reflecting the gut-kidney axis. Specifically, fecal suspension samples from patients with CKD1 (n = 12) and CKD4 (n = 17) were analysed by LC-MS/MS, using both the Human and Bacterial UniProt RefSeq reviewed databases.

SUBMITTER: Lohia S 

PROVIDER: S-EPMC11226915 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature