DNA choreography: correlating mobility and organization of DNA across different resolutions from loops to chromosomes.

ABSTRACT: The dynamics of DNA in the cell nucleus plays a role in cellular processes and fates but the interplay of DNA mobility with the hierarchical levels of DNA organization is still underexplored. Here, we made use of DNA replication to directly label genomic DNA in an unbiased genome-wide manner. This was followed by live-cell time-lapse microscopy of the labeled DNA combining imaging at different resolutions levels simultaneously and allowing one to trace DNA motion across organization levels within the same cells. Quantification of the labeled DNA segments at different microscopic resolution levels revealed sizes comparable to the ones reported for DNA loops using 3D super-resolution microscopy, topologically associated domains (TAD) using 3D widefield microscopy, and also entire chromosomes

SUBMITTER: Pabba MK 

PROVIDER: S-EPMC11227476 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature