16S rRNA, metagenomics and 2bRAD-M sequencing to decode human thanatomicrobiome.

ABSTRACT: Microorganisms are essential in the decomposition of corpses and play a significant role in forensic science. However, previous studies have primarily focused on animal remains, specifically the gut, skin, and burial environment. Insufficient research has been conducted on the microbiota of human cadavers, especially in cases of advanced decomposition and additional tissues, resulting in a lack of relevant reference data. In this study, the microbiota of eight cadavers at different stages of decomposition were detected using 16S rRNA, metagenomic sequencing and 2bRAD-M sequencing. Nine different sites, including oral and nasal cavities, heart, liver, spleen, lung, kidney, muscle and gut, were analysed and the efficacy of these methods was evaluated. The results showed that 16S rRNA sequenc

SUBMITTER: Huang X 

PROVIDER: S-EPMC11227556 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature