Engineered droplet-forming peptide as photocontrollable phase modulator for fused in sarcoma protein.

ABSTRACT: The assembly and disassembly of biomolecular condensates are crucial for the subcellular compartmentalization of biomolecules in the control of cellular reactions. Recently, a correlation has been discovered between the phase transition of condensates and their maturation (aggregation) process in diseases. Therefore, modulating the phase of condensates to unravel the roles of condensation has become a matter of interest. Here, we create a peptide-based phase modulator, JSF1, which forms droplets in the dark and transforms into amyloid-like fibrils upon photoinitiation, as evidenced by their distinctive nanomechanical and dynamic properties. JSF1 is found to effectively enhance the condensation of purified fused in sarcoma (FUS) protein and, upon light exposure, induce its fibrilization. We

SUBMITTER: Chuang HY 

PROVIDER: S-EPMC11227587 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature