Hyperpolarized [1-¹³C]Acetyl-l-Carnitine Probes Tricarboxylic Acid Cycle Activity In Vivo.

ABSTRACT: Mitochondrial oxidative phosphorylation (OXPHOS) is sensitive to a variety of biological factors, and dysregulated OXPHOS is observed during the development of numerous pathological conditions. ATP production via OXPHOS is intrinsically dependent on the availability of acetyl-coenzyme A (CoA), which can enter the tricarboxylic acid (TCA) cycle to drive the oxidative pathway. Acetyl-l-carnitine (ALCAR) is an interchangeable endogenous source of acetyl-CoA, and therefore, ALCAR-derived probes are uniquely positioned for the assessment of OXPHOS. In this report, we develop hyperpolarized (HP) [1-¹³C]ALCAR as a noninvasive probe to investigate cardiac TCA cycle activity in vivo. We initially synthesized the isotopically labeled substrate and demonstrated that the ¹³

SUBMITTER: Chen J 

PROVIDER: S-EPMC11227661 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature