Distraction force promotes the osteogenic differentiation of Gli1⁺ cells in facial sutures via primary cilia-mediated Hedgehog signaling pathway.

ABSTRACT:

Background

Trans-sutural distraction osteogenesis (TSDO) involves the application of distraction force to facial sutures to stimulate osteogenesis. Gli1⁺ cells in the cranial sutures play an important role in bone growth. However, whether Gli1⁺ cells in facial sutures differentiate into bone under distraction force is unknown.

Methods

4-week-old Gli1ER/Td and C57BL/6 mice were used to establish a TSDO model to explore osteogenesis of zygomaticomaxillary sutures. A Gli1⁺ cell lineage tracing model was used to observe the distribution of Gli1⁺ cells and explore the role of Gli1⁺ cells in facial bone remodeling.

Results

Distraction force promoted bone remodeling during TSDO. Fluorescence and two-photon scanning image

SUBMITTER: Jin M 

PROVIDER: S-EPMC11227703 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature