Rankl genetic deficiency and functional blockade undermine skeletal stem and progenitor cell differentiation.

ABSTRACT:

Background

Skeletal Stem Cells (SSCs) are required for skeletal development, homeostasis, and repair. The perspective of their wide application in regenerative medicine approaches has supported research in this field, even though so far results in the clinic have not reached expectations, possibly due also to partial knowledge of intrinsic, potentially actionable SSC regulatory factors. Among them, the pleiotropic cytokine RANKL, with essential roles also in bone biology, is a candidate deserving deep investigation.

Methods

To dissect the role of the RANKL cytokine in SSC biology, we performed ex vivo characterization of SSCs and downstream progenitors (SSPCs) in mice lacking Rankl (Rankl^-/-) by means of cytofluorimetric sorting and analysis of SSC populations fro

SUBMITTER: Schiavone ML 

PROVIDER: S-EPMC11227705 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature