New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis.

ABSTRACT:

Background

Bone marrow-derived mesenchymal stem cell (_BMMSC)-based therapy has become a major focus for treating liver fibrosis/cirrhosis. However, although these cell therapies promote the treatment of this disease, the heterogeneity of _BMMSCs, which causes insufficient efficacy during clinical trials, has not been addressed. In this study, we describe a novel Percoll-Plate-Wait procedure (PPWP) for the isolation of an active cell subset from _BMMSC cultures that was characterized by the expression of neuroglial antigen 2 (NG2/_BMMSCs).

Methods

By using the key method of PPWP and other classical biological techniques we compared NG2/_BMMSCs with parental _BMMSCs in biological and functional characteristics within

SUBMITTER: Hu D 

PROVIDER: S-EPMC11227708 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature